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Proteomics

In the past four years we have made a substantial investment in Proteomics technology with the purchase of state of the art instrumentation. Rowett now runs a state-of-art laboratory for protein isolation and identification. This investment has been backed up by our highly qualified and experienced staff which brings together a service of international standing. The quality of the Rowett proteomics service has recently been recognised by our inclusion a a group of only 50 laboratories worldwide recommended by Bio-Rad.

The Proteomics Unit offers a service to research and commercial clients who have a low or high throughput requirement for electrophoretic separation of proteins and their subsequent identification by peptide mass fingerprinting by MALDItof and nano-flow HPLC combined with ESI mass spectrometry.

Following the separation of sample proteins on 2D gel electrophoresis the gel images are analysed for differences in protein expression brought about by nutritional status, experimental manipulations or disease states. Image analysis software is networked throughout the Institute allowing multiple access to the software. Protein spots of interest are excised from relevant gels using a robotic spot cutter and tryptic peptides generated from these samples using a multi-tasking liquid handling robot. The tryptic peptides generated are subjected to MALDItof (see mass spectrometry section) for peptide mass fingerprinting and identification of proteins by submission of peptide masses for comparison to various protein databases. For more information on each of these services follow the links below. To discuss your specific requirements and receive a quote contact us.

Instruments available:

• BioRad IEF cells
• IsoDalt & BioRad Dodeca cell large format electrophoresis tanks
• BioRad PD Quest Image analysis software (networked)
• BioRad spotcutter (compatible with PD Quest)
• MassPrep Station (robotic liquid handling)
• ABI Voyager DE PRO MALDI tof mass spectrometer
• Applied Biosystems Q Trap