Link to RRI

BioRad IEF cells

Separation of proteins is achieved in this instrument using a pH gradient immobilised on a plastic strip (IEF strips) on the basis of their isoelectric points.

Isodalt & BioRad Dodeca cell large format electrophoresis tanks

Proteins separated on IEF strips are separated in a second dimension by SDS polyacrylamide gel electrophoresis and are stained with mass spectrometry compatible Coomassie Blue.

BioRad spotcutter (compatible with PD Quest)

Linked to the PDQuest software is the spotcutting robot which excises the spots from the gel of interest.

MassPrep Station (robotic liquid handling)

Protein spots are chemically modified and ultimately cleaved using trypsin an enzyme which cleaves proteins at specific amino acid residues in the polypeptide chain. This generates a mixture of peptides whose masses can act as a “fingerprint” of the parent protein.

ABI Voyager DE PRO MALDI tof mass spectrometer

The MALDI tof mass spectrometer measures the masses of the tryptic peptides generated by the MassPrep station. Matching these peptide masses against a database provides protein matches which using bioinformatic packages can provide information about the protein of interest in a particular biological process. Further information about proteins which cannot be identified by MALDItof can be obtained by submission of the tryptic peptides to nanoflow HPLC and ESI mass spectrometry using the ABI Q Trap.

Applied Biosystems Q Trap

Ionisation
Electrospray Ionisation (ESI)
Turbospray Source
NanoSpray Source

LC/MS, LC/MS/MS and LC/MS³ capability

Preparative Systems
LC Packings Nanoflow LC System
On line data base searching
De novo sequencing

To discuss you specific requirements in more detail and for quotations please contact us ( link to contact page )